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Dead Cell Removal

Dead Cell Removal

ClioCell Nanoparticles are designed to deplete non-viable (dead or dying) cells from eukaryotic cell cultures. ClioCell Nanoparticles selectively bind and remove non-viable cells via ligands exposed on dead and dying cells, in a range of different media and temperatures conditions – the resulting cell preparation remaining after ClioCell treatment is a highly viable population of cells, unhindered by the deleterious signalling effects caused by neighbouring apoptotic and necrotic cells (slow cell growth, loss of reproducibility, decreased purity, less yield).

Key benefits: 

  • Cost-effective
  • Simple to use
  • Efficient
  • Disposable 
  • Scalable
  • GMP compatible
Removing dead and dying cells from cell culture preparations has a number of beneficial effects for many protocols, including:
  • Antibody Production: Monoclonal antibody production in ClioCell-treated cultures is almost double that of control cultures (mAbs 1:4, 370-376; July/August 2009; Landes Bioscience]
  • Embryonic Stem Cells: Growth curves significantly improved after ClioCell treatment (9x106 cells vs. 6x106 cells after 7 days of culture)
  • Drug Screening: Chemotaxis assay sensitivity increased 170% after ClioCell treatment
  • Cell Revival: Re-establishment of cell populations after cryopreservation is vastly increased after ClioCell treatment to remove cells damaged from freezing – when BL2 cells are frozen for 24 h, then cultured for 5 h in BL2 medium, 90% of cells in the population treated with ClioCell are viable (as determined by FACS analysis), compared to 30% in the non-treated population. The reduction of dead cells speeds proliferation, and is a useful tool for optimising cell growth in manufacturing conditions.
  • Cell therapy: Avoid an inconsistent supply of product due to apoptotic/necrotic cells – ClioCell allows a reduction in manufacturing cycle (lower CoGs), enhanced purity and potency (regulatory and quality), increased shelf-life and greater  efficacy
Kit components:
  • Nanoparticles (250 nm)
  • Yield Enhancing Separation (YES) medium
  • Magnet

» Click here to view the Dead Cell Removal products