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Cell Free Protein Expression

5 PRIME RTS Cell free Protein Expression


RTS 100 E.coli Disulfide Kit

The only in vitro protein expression system that can express properly folded disulfide-bonded proteins that cannot be produced under reducing conditions

  • Rapid parallel synthesis of disulfide-bonded proteins
  • Rapid functional testing of different constructs (e.g., for epitope or functional domain mapping)
  • Expression of toxic gene products
  • Production of proteins in a wide molecular weight range
  • Optimization of expression conditions (chaperones, detergents, temperature, reaction time, etc.).

 


» Click here to view the RTS 100 E.coli Disulfide Kit products



RTS 100 E.coli HY Kit

Small-scale expression with yields of up to 20 µg of protein for screening templates and optimizing expression conditions

Features and Benefits

  • Compatibility with PCR-generated templates and plasmids
  • Rapid functional testing of PCR-generated mutations
  • Synthesis of truncated gene products from PCR-generated or restriction enzyme digested DNA, for epitope or functional domain mapping
  • Expression of toxic gene products
  • Rapid high throughput protein synthesis reactions with easy scale-up


» Click here to view the RTS 100 E.coli HY Kit products



RTS Amino Acid Sampler

Amino acid specific protein labeling for structure determination

Features and Benefits

  • The protein of interest is expressed from an exogenous plasmid template and is the only protein produced and labeled.
  • The translation reaction is performed in a small volume, requiring relatively low amounts of labeled amino acids.
  • The metabolism of amino acids is dramatically lower compared to cellular systems, leading to the most effective amino acid-specific labeling. Labeled amino acids that cannot be specifically introduced in proteins expressed in E. coli can easily be incorporated in proteins using RTS (e.g., specific labeling of histidine).
  • No expensive media are required since cells do not require cultivation

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» Click here to view the RTS Amino Acid Sampler products



RTS GroE Supplement 5 vials for five 1ml reactions

A tailor-made chaperone mix for optimisation of protein expression in RTS

Features and Benefits

  • Enhance solubility and functional activity of proteins expressed with RTS
  • Easily combine with RTS 100 and 500 E. coli HY platforms, using an optimised protocol
  • Improve experimental results.


» Click here to view the RTS GroE Supplement 5 vials for five 1ml reactions products



RTS pIVEX His-tag 2^nd Gen. Vector Set

Vectors for preparative scale cell-free protein expresion in E. coli lysates

Features and Benefits

  • Hexa-histidine fusion tag allows easy detection and purification of the expressed protein
  • Regulatory elements for optimzed expression in RTS E. coli system
  • Stable template for preparative scale protein expression

 


» Click here to view the RTS pIVEX His-tag 2^nd Gen. Vector Set products



RTS Wheat Germ LinTempGenSet His6-tag

Generation of PCR products for in vitro protein expression using the RTS 100 Wheat Germ CECF Kit.

Features and Benefits

  • This method for protein expression avoids the time-consuming step of cloning into T7-expression plasmids.
  • The provided PCR templates contain sequences that allow either N-terminal or C-terminal His6-tagging


» Click here to view the RTS Wheat Germ LinTempGenSet His6-tag products



RTS100 wheatgerm CECF Kit

RTS Wheat Germ system for eukaryotic protein synthesis system using the continuous-exchange, cell-free (CECF) technology

  • Achieve scalable and preparative eukaryotic cell-free protein expression.
  • Express eukaryotic proteins with high success rates, generally without sequence optimisation.
  • Maximise the production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C.
  • Avoid cloning; express your protein directly from PCR products; or utilise vectors or mRNA as template.
  • Enable expression of toxic proteins that cannot be produced in cellular or bacterial expression systems.


» Click here to view the RTS100 wheatgerm CECF Kit products